Effects of Aerobic Exercise Combined with Resveratrol Therapy on the Myocardial Cell Apoptosis Factors TNF-α and Caspase-3 in Rats with Type-II Diabetes Mellitus
Objective: The objective is to investigate the effects of resveratrol on tumor necrosis factor alpha (TNF-α) and cysteine aspartate proteolytic enzyme-3 (Caspase-3) in myocardial cells of rats with type-II diabetes mellitus, and the intervention effects of resveratrol combined with aerobic exercise on rats with type-II diabetes mellitus. Methods: A total of 36 SPF male Sprague-Dawley (SD) rats aged 8-10 weeks were purchased from XX Laboratory Animal Center and were selected as research objects. After a week of adaptive feeding, all rats were randomly divided into 4 groups, which were respectively the blank group, the model group, the therapy group, and the therapy+exercise group, with 9 rats in each group. Rats in the blank group were not subjected to any interventions, and rats in the other 3 groups were constructed as models of type-II diabetes mellitus. Then, rats in the therapy group were treated with resveratrol by gavage for 6 consecutive weeks; based on the resveratrol administration, rats in the therapy+exercise group underwent aerobic exercise for 6 consecutive weeks. At the end of the experiment, the fasting plasma glucose (FPG), serum insulin (FINS), homeostasis models of insulin resistance, and cardiac function indicators of rats in each group were compared; in addition, the structures of myocardial tissues of rats in each group were observed and compared under a microscope to analyze the changes in the protein expressions of TNF-α and Caspase-3 in myocardial tissues of rats in each group. Results: 1. After 6 weeks of treatment, in the resveratrol therapy group and the therapy+exercise group, the FPG levels of rats were respectively (11.91±2.86) mmol/L and (7.94±1.55) mmol/L, which were obviously decreased compared with the model group, in which the decline of FPG in the therapy+exercise group was more obvious (P<0.05); in addition, the FINS levels and homeostasis models of insulin resistance in the resveratrol therapy group and the therapy+exercise group showed a downward trend, in which the downward trend of the therapy+exercise group was more obvious (P<0.05). 2. The myocardial tissue structures of rats in the resveratrol therapy group and the therapy+exercise group were obviously improved compared with the model group; the arrangement of myocardial cells was relatively regular, the infiltration of inflammatory cells was less, the aggregation of nuclei was obviously reduced, and the cardiac function indicators were obviously improved (P<0.05). 3. The expression levels of TNF-α protein and Caspase-3 protein in the myocardial tissues of rats in the resveratrol therapy group and the therapy+exercise group were obviously decreased, in which the decreasing effects in the therapy+exercise group were better than those in the resveratrol therapy group (P<0.05). Conclusion: Aerobic exercise in combination with resveratrol could reduce the expression levels of TNF-α and Caspase-3 in myocardial tissues of rats with diabetes mellitus, thereby reducing the expressions of apoptosis factors in rat myocardial tissues and improving the pathological changes in the myocardial tissues of rats with diabetes mellitus; in addition, the therapeutic effects of the combination of aerobic exercise and resveratrol were better than the single therapy of resveratrol, which was worthy of clinical reference and application.