Effects of Exercise Training on Lipid Peroxidation and Lipofuscin Content in Aging Rats

  • Qi Huang

Abstract

The purpose of this paper is to use the composite preparation as an antioxidant, and combine exercise training to observe their effects on the antioxidant capacity of aging mice, and observe the effect on aging with lipofuscin as an indicator. In this paper, 18 mice were selected, aged 14 months, male and female, weighing 30-40 grams, randomly divided into three groups: saline group (n=6), medication group (n=6), medication combined with training group ( n=6), group caged group A was intragastrically administered with saline 0.2ml/day, group B was given 0.2ml/day with compound preparation, group C was given 0.2ml/day with compound preparation, while swimming in pool, swimming The time starts from 5 min/d, increasing by 30 s every day until 10 min/d, and the water temperature is controlled from 25 °C to 28 °C. The above process was carried out continuously for 40 days. The animals were sacrificed by cervical dislocation. The myocardium and brain were obtained and immersed in physiological saline for 20 min. The blood in the tissue was squeezed out with a glass rod, washed, and the surface moisture of the tissue was blotted with filter paper. 0.175 mol/L KCL-Tris-HCL buffer (pH=7.4) was added at W/V=1:9, and homogenized by homogenizer in an ice bath, and centrifuged at 2000 r/min for 10 min to take the supernatant for trial. The sample was quantitatively determined according to the Bradford method, and the OD value at the wavelength of 595 nm was measured on a spectrophotometer, and the standard curve was used to obtain the protein content of the sample. The test results show that the myocardial metabolism is strong, the consumption of O2 is more, the free radicals are more, and the LPO level is also high, therefore, whether the addition of antioxidants or combined training of myocardial LPO levels can be significantly reduced. Lipofuscin is a product of peroxidation of cellular lipid unsaturated fatty acids. This substance contains abnormal bonds, and it is considered to be the basic feature of cell aging as the age and free radical levels increase. The drug and combined training group the levels of lipofuscin in the myocardium and brain decreased with the decrease in LPO levels and the use of antioxidants. There was no significant decrease in LPO levels in the brain of the medication group, but the level of lipofuscin was significantly decreased.

Published
2019-08-04