Determination of Clenbuterol Residues in Animal Foods and Biomaterials by Gas Chromatography-Mass Spectrometry

  • Xifeng Yan

Abstract

The purpose of this paper is to determine the clenbuterol residues in animal foods and biomaterials by gas chromatography-mass spectrometry. This paper establishes a method for the determination of four β-agonist residues by GC/MS. Through the evaluation of the linearity, accuracy, precision, detection limit, quantitative limit and reliability of the method, the method of this paper fully satisfies the requirements of the residual method. Through the investigation and optimization of various factors, the optimal analysis conditions are selected, and the traditional processing methods are simplified, making it simple, economical and suitable for a large number of sample detection. The test results show that the method meets the requirements for the confirmation of such drugs in China, the European Union and the United States. It is also applicable to the detection of four β-agonists. It is qualitatively and quantitatively completed at one time, a reliable and rapid method for quantification and confirmation of agonist drug residues. The uncertainty of the method for determining the β-agonist residues in pork by gas/mass spectrometry was studied for several influencing factors (measurement repeatability, sample uniformity, standard solution, and extraction process) mainly involved in the residue analysis. Evaluation, it is considered that in the process of evaluating the uncertainty of the residual detection method, the detection method and the uncertainty caused by the detection of dispersion should be mainly considered, and the uncertainty caused by each calculated component is much smaller than the former and can be ignored.

Published
2019-08-04