Metabolism of Mineral Elements in Fat and Liver and Intervention of Swimming Exercise in High-fat Diet Mice

  • Dongyong Zhao
Keywords: Diet Mice, Obesity Model, Blood Lipid, Liver Mineral Elements, Exercise Intervention


In order to study the metabolism of mineral elements in fat and liver in mice fed with high-fat diet
(HD) after swimming intervention, 45 male Specific Pathogen Free (SPF)-grade Sprague-Dawley (SD) mice
were selected as research objects. They were divided into quiet control group (C), HD group, and high-fat diet
exercise (HDE) group. After that, the mice in each group were tested for body weight and blood lipid, and their
liver tissues were collected for hematoxylin (HE) staining and mineral element analysis, and the data were
collected and counted. The results showed that the weight of mice in the HD group was significantly heavier
than that in the control group, and the weight of mice in the exercise intervention group was significantly lower
than that in the HD group, with statistical difference (P < 0.05). In the detection and analysis of blood lipids, the
concentrations of the serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL-C) in the
HD group were higher than those of the control group. After exercise intervention, the concentration decreased,
while that of high-density lipoprotein (HDL-C) decreased in the HD group, and increased after the exercise
intervention (P < 0.05). In the HE staining analysis of liver tissue, it was found that the arrangement of
hepatocytes was regular after exercise intervention, and no inflammatory necrosis or fibrous tissue proliferation
was found. In the analysis of liver minerals, zinc, copper, iron, calcium, magnesium, and manganese recovered
after exercise intervention, with statistical difference (P < 0.05). Therefore, through this study, it is found that
exercise intervention in mice has a good mitigation effect on the body weight and liver mineral element
metabolism disorders, and achieves the desired effect. Although there are some shortcomings in the research
process, it still provides experimental basis for the treatment of obesity-related symptoms in the later period, and
has important research significance.