Exhaustive Swimming on Myocardial Mitochondrial Ultrastructure in Mice

  • Qing Tang
Keywords: Ultrastructure Analysis, Mitochondrial interior, Energy Conversion, Electrochemical Gradient

Abstract

So far, scientists at home and abroad have used electron microscopy to study the changes of
myocardial ultrastructure after exercise. However, most past studies have only used the methods of qualitative
or simple quantitative. The purpose of this paper is to investigate the changes of mice’s myocardial
mitochondrial ultrastructure during exhaustive swimming. Earlier, there were only a few reports on changes of
mitochondrial submicroscopic structure. This experiment aims to study some areas that were less involved
previously. Sixty mice grown in the animal breeding laboratory center and let they stay in the laboratory for 2
weeks, feeding them with various nutrients and concentrates. Two weeks later, conducted the swimming ability
test. Selected five rats as the calm normal control group randomly, 15 were loaded with 10% body weight in the
tail, swimming to be exhausted, choose 5 samples randomly, placed them in the depletion group, and 5 samples
were recovered group after 24 hours of body recovery, and the remaining 5 died of swimming, they were
super-exhaustive group and take them to the test bed for treatment quickly after death. Observation under a
transmission microscope, performed sample preparation immediately after completion of a series of experiments,
and carried out the comprehensive analysis by recording experimental process data. The experimental results
show that ions in the mitochondria produce energy along the electrochemical gradient, and the mitochondrial
membrane can convert this energy into ATP for re-synthesis. The experimental results show that mitochondria
participate in energy conversion, its increase and decrease can affect the efficiency of energy conversion, so the
number, volume and shape of mitochondria can change with the change of energy metabolism.

Published
2019-09-01