Effects of Vasoactive Peptide on Blood Pressure, Immune Cell Population and Cytokines in Spontaneously Hypertensive Rats Fed with High Salt Diet

  • Xuan Zhou
Keywords: vasoactive peptide, high salt diet induced spontaneous hypertension, blood pressure, immune cell population, cytokine

Abstract

To investigate the effect of vasoactive peptide on blood pressure, immune cell population and
cytokines in spontaneously hypertensive rats fed with high salt diet. Forty-eight male spontaneously
hypertensive rats were randomly divided into four groups, including control group (n=12), model group (n=12),
fosinopril group (n=12) and urocortin group (n=12). The control group was given intragastric administration of
the same dose of normal saline, the model group was given 0.01 ml 0.9% sodium chloride/ (g·d), the fosinopril
group was given 4 mg / (kg·d), and the urocortin group was given 3 μg / (kg·d), once a day for 8 weeks. The
changes of the systolic blood pressure of the rat caudal artery before administration, 4 weeks and 8 weeks after
administration were detected by non-invasive blood pressure instrument; the changes of T lymphocyte subsets in
peripheral blood were detected by flow cytometry; the levels of IL-1 β, IL-6 and TNF -α were measured by
enzyme-linked immunosorbent assay. The systolic blood pressure of the caudal artery in the fosinopril group
and urocortin group was lower than that in the model group at 4 weeks and 8 weeks after administration, which
was lower in urocortin group than fosinopril group (P < 0.05). CD3 + CD4 + in two groups were lower than that
in model group, and CD3 + CD8 + and CD4 + CD25 + were higher than that in model group (P < 0.05); CD3 +
CD4 + in urocortin group was lower than that in fosinopril group, while CD3 + CD8 + and CD4 + CD25 + were
higher than that in fosinopril group (P < 0.05). The levels of IL-1 β, IL-6 and TNF-α in fosinopril group and
urocortin group were lower than those in the model group (P < 0.05); the levels of IL-1 β, IL-6 and TNF-α in
urocortin group were lower than those in fosinopril group (P < 0.05). HE staining showed that the endothelial
cell damage of basilar artery, glomerular atrophy and vascular wall thickening in the model group were more
than those in the control group; after fosinopril and urocortin intervention, the endothelial cell damage of basilar
artery, glomerular atrophy and vascular wall thickening were alleviated. Vasoactive peptide can reduce the blood
pressure, regulate the changes of T-lymphoid immune cell population and reduce the inflammatory response of
spontaneously hypertensive rats fed with high salt diet.

Published
2019-11-01