Effect of IKK Complex Regulating NFKB Pathway on Proliferation of Pig Intestinal Epithelial Cells

  • Pengfei Gao
Keywords: IKK complex regulation, NFKB pathway, IKK beta, cell proliferation


Rel/NFKB family of eukaryotic nuclear transcription factors exists widely in cells from insects to
humans and is involved in cell differentiation, development, apoptosis, adhesion and inflammatory responses.
Generally, NFKB release binds to its inhibitory protein IKB release and remains inactive in the cytoplasm.
When various external signals act on cells, activated NFKB enters the nucleus and performs its functions. At
present, the signal transduction mechanism of NFKB activation has been basically clarified, and IKB Kinase
(IKK) plays an important role in it. The purpose of this study was to investigate the effect of IKK complex
regulation of NFKB pathway on the proliferation of pig intestinal epithelial cells. In this paper, the structure and
function of pig genes were analyzed and predicted by bioinformatics. PcDNA3.1 + vector were constructed to
obtain ipec-j2 stable cell lines regulated by IKK complex expression. The effect of IKK complex regulated
expression on ipec-j2 cell proliferation was detected by cell count and MTT, and the expression level of key
proteins in the NKFB signaling pathway was detected by Western blot. To analyze the mechanism of promoting
intestinal epithelial cell proliferation in pigs. In this study, changes in the expression of key proteins in the
NKFB signaling pathway after IKK complex regulation was over expressed in ipec-j2 cells were detected. The
results showed that the expression levels of Axin2 and gsk-3 beta proteins were significantly decreased (P<0.05),
while the expression levels of beta-catenin, c-myc and cyclinD1 proteins were significantly increased (P<0.05),
indicating that IKK complex regulation could promote the proliferation of pig intestinal epithelial cells by
activating NKFB signaling pathway.