Anti - infection Immunity of Mice after Exhausted Exercise

  • Yanming Pei
Keywords: Exhausted exercise, T lymphocytes, Anti - infection immune function, Animal model

Abstract

In order to explore the effect of exhausting exercise on the anti-infection immune function of human
body and to provide experimental support for promoting reasonable exercise. A mouse model of exhausted
exercise was established by using a treadmill. The contents of white blood cells and neutrophils in mice before
and after exhausting exercise were measured by cell counter, so as to evaluate the changes of immune system
related immune cells in mice. In vitro cell culture technique was used to calculate the conversion rate of T
lymphocytes in mice before and after exhaustion exercise, and explore the effect of exhaustion exercise on T
cell function in the immune system of mice. The content of Tregs in the blood of mice was detected by
enzyme-linked immunosorbent assay (ELISA) to research the effect of exhausting exercise on regulatory T cells
in mice. Mice in the exhausted exercise group and the normal group were injected with bacillus shiga by gavage
to observe the physiological activities of mice in different groups and analyze the anti-infection ability of mice
after exhausted exercise. The results showed that the mouse treadmill could successfully establish the exhausted
exercise animal model and lay a foundation for the subsequent experiment. White blood cells and neutrophils in
the exhausted exercise group were significantly different from those in the normal group (P<0.01). The T cell
transformation experiment showed that the T cell conversion rate in the exhausted exercise group decreased
significantly (P<0.01). ELISA data showed that Tregs content in blood of the exhausted exercise group
increased significantly (P<0.05). After bacterial transfection, the mice in the exhausted exercise group showed
obvious malaria symptoms. The results showed that strenuous exercise may lead to abnormal immune system,
decreased anti-infection ability. It also made mice more susceptible to invasion by bacteria and parasites; At the
same time, excessive exercise inhibited the transformation function of T lymphocytes in mice, regulating the
expression of T cells. An effective experimental basis is provided for sports medicine and a reasonable exercise
guidance for public fitness.

Published
2019-11-01