Effects of Aerobic Exercise at Different Intensities on Rats’ Hepatocytes

  • Xiaofeng Shi
Keywords: Aerobic Exercise, Hepatocytes, Apoptotic Factors, Western Blot, ER Stress Protein


In order to study the effects of aerobic exercise at different intensities on rats’ hepatocytes, 30 SPF
(Specific Pathogen Free)-grade SD (Sprague-Dawley) male rats were selected as experimental subjects, and
were divided into quiet control group, moderate aerobic exercise group, and overload exercise group. After that,
the weight of rats in each group was measured, and their liver tissues were collected for HE (Hematoxylin-Eosin)
staining analysis. TdT-mediated dUTP Nick-End Labeling (TUNEL) detection and Western blot method were
used to detect the apoptotic level of hepatocytes, and the data were collected and counted. The results showed
that the hepatocytes in the quiet control group were the most complete with large and round nuclei. The cell
outline of moderate aerobic exercise group was changed, and the cell gap was increased significantly. The
hepatocytes in the overload exercise group were irregular, and the cytoplasm was swollen and the nucleus
showed pyknosis. The apoptotic effect was further enhanced, and it was found that the positive expression rate
of nucleus increased significantly with the increase of exercise intensity. Western blot analysis of related factors
in rat hepatocytes showed that the expression of Bax (BCL2-Associated X) and ER (endoplasmic reticulum)
stress protein increased with the increase of exercise intensity in rats, with significant statistical difference (P <
0.01). The expression level of Bcl-2 (B-cell lymphoma-2) decreased with the increase of exercise intensity in
rats (P < 0.01). Therefore, through this study, it is found that after different intensities of exercise intervention
on rats, the expression levels of Bax, Bcl-2, and ER stress protein and other related apoptotic factors in
hepatocytes changed, which further led to changes in apoptotic level. The greater the intensity of exercise was,
the faster the metabolism was, and it achieved the desired effect. As a result, although there are some
shortcomings in the research process, it still provides experimental basis for the mechanism of the effect of
exercise intensity on hepatocytes in the later period, and has important research significance.