Pulmonary Fibrosis Model Induced by Repeated Intraperitoneal Administration of Paraquat in Mice

  • Yunhua Liu
Keywords: Paraquat Death, Low-Dose Intraperitoneal Administration, Pulmonary Fibrosis, Lung Injury

Abstract

The purpose of this paper is to establish a mouse model of lung injury and pulmonary fibrosis
induced by paraquat (PQ), to explore the mechanism of batrogen-induced pulmonary fibrosis induced by
fibromycin, and to provide theoretical basis for its clinical application. A more ideal animal model is laid to lay
the foundation. Methods Male C57BL/6J (C57) mice (n=30) aged 8-10 weeks were randomly divided into blank
group, paraquat group, paraquat + bleomycin group, and Elisa. Methods The expressions of TGF-β1, a-SMA,
Smad3 and Smad2 in plasma were detected on the 1st, 3rd, 7th, 14th and 21st day after exposure. The paraquat
group was intraperitoneally injected with PQ10mg/kg and injected five times. Three days of injection, paraquat
+ bleomycin group intratracheal injection of bleomycin 3mr / kg. On the 7th, 14th, and 28th day after successful
modeling, 10 rats in each group were used to measure the general condition, body mass and pathological
changes of lung tissue to calculate the lung coefficient, observe the histological changes of rats, and measure the
lungs of rats. Expression of hydroxyproline, superoxide dismutase (SOD), malondialdehyde (MDA) and KL-6
mucin. The statistical data SPSS21.0 was used for statistical analysis of the collected data. The comparison
between the two groups was performed by Mann-Whitney U test. Experiments have shown that PQ
intraperitoneal injection and tracheal injection of BLM can cause lung injury and fibrosis model. By comparing
the increase in body mass, mental status, pathological changes of lung tissue, and rate of formation at different
time points in the control group, it was shown that repeated small doses of intraperitoneal injection of PQ were
compared with conventional bleomycin intratracheal injection. The mold is more convenient and effective, and
the success rate is higher. Bleomycin can induce paraquat-induced pulmonary fibrosis by inhibiting the
expression of TGF-β1, a-SMA and Smad3, Smad2 proteins, and repeated low-dose intraperitoneal injection of
PQ is a more ideal modeling method. The method induced bymycin has a higher success rate.

Published
2019-11-01