Effects of Hypoxia Recovery on Rat Skeletal Muscle Focal Adhesion Complex after One-Time Eccentric Exercise

  • Xinglin Wei
Keywords: Skeletal Muscle, Hypoxic Exercise, Plaque Complex, Plaque Kinase

Abstract

Overtraining or maladaptive heavy exercise has a negative impact on skeletal muscle, resulting in
ultrastructural changes of muscle fibers, resulting in injury. Movement induced mechanical stimulation regulates
gene expression through the physical connection between the cytoskeleton and the nuclear matrix. The purpose
of this study was to investigate the effects of different recovery time and recovery conditions on protein content
of skeletal muscle adhesion complex after a centrifugal exercise. The experimental scheme was that 56 male SD
rats aged 8 weeks were randomly divided into the quiet control group, the normal oxygen recovery group after
exercise and the hypoxia recovery group. The hypoxia recovery group received one-time high-intensity
centrifugal exercise and hypoxia (12.7%O2) intervention, while the normal oxygen recovery group received
exercise intervention. Conclusion: compared with the control group, the expression of integrin  7 in the
normal oxygen recovery group was significantly increased at 24h (P<0.05), and gradually recovered to the
protein level of the control group with the extension of time, and the expression of integrin  7 in the hypoxic
recovery group was also significantly increased at 72h (P<0.01). The expression of skeletal muscle integrin  1
in the normal oxygen recovery group and the hypoxic recovery group was significantly higher than that in the
hypoxic recovery group (P<0.05, P<0.01). Moreover, p-fak expression in skeletal muscle of rats in the normal
and hypoxic recovery groups decreased with time, and the expression in the hypoxic recovery group was higher
than that in the normal oxygen recovery group, among which the expression in the 48h and 72h groups was
significantly lower than that in the control group (p <0.05). P-paxillin decreased first and then increased in the
normoxic recovery group and hypoxic recovery group. Except for the 24h group in the normoxic recovery group,
all the other groups were significantly lower than the control group (p <0.05).

Published
2019-11-01