Effects of C-Type Natriuretic Peptide on In Vitro Maturation of Goat Oocyte and Parthenogenetic Embryo Development

  • Yanjun Zhang
Keywords: C-Type Natriuretic Peptide, Oocyte Cells, CNP Pretreatment, Goat Ovary


During the in vitro maturation of mammalian oocyte, the concentration of cyclic adenosine
monophosphate (cAMP) in the oocyte decreases rapidly due to the isolation from the follicular environment in
vivo, which leads to premature recovery of meiosis and complete nuclear maturation, and ultimately leads to the
nuclear cytoplasm of the oocyte. Maturity is not synchronous, which affects the subsequent development ability
of oocyte. So far, many substances have been used to induce synchronous maturation of nuclear and cytoplasm
in vitro matured oocytes, but most of them cannot achieve the purpose of both inducing synchronization of
nuclear and cytoplasmic maturation and improving the quality of mature oocytes. C-type diuretic natriuretic
peptide (CNP) is a highly conserved small molecule polypeptide, which widely exists in mammals. Studies have
shown that CNP can inhibit meiotic recovery of mouse and porcine oocytes, and CNP is expressed in granulosa
cells of goat follicular cells. However, the expression of C-type natriuretic peptide receptor 2 (NPR2) in goat
ovaries and the effects of CNP on meiosis and maturation quality of mature oocytes in vitro have not been
reported. The effects of CNP pretreatment on meiotic arrest and subsequent development of ovine oocyte were
studied. The effects of CNP on the expression of genes related to maternal and cumulus cell amplification
before and after oocyte maturation confirmed that CNP could not only inhibit the recovery of mouse oocyte
decrement, but also affect the expression of key genes during in vitro maturation.