Resveratrol Protects the Ovarian Function of Rats with Premature Ovarian Dysfunction by Activating Pi3kaktmtor Signaling Pathway and Antioxidant Stress Mechanism

  • Jinyu Xia
Keywords: Resveratrol, PI3K / Akt / mTOR Signaling Pathway, Antioxidant Stress, Premature Ovarian Dysfunction

Abstract

To investigate the protective effect of resveratrol (RES) on the ovarian function of rats with
premature ovarian dysfunction by activating the signal pathway of phosphatidylinositol 3-kinase (PI3K) /
protein kinase B (Akt) / mammalian rapamycin target protein (mTOR) and the mechanism of antioxidant stress.
A total of 95 female SD rats were randomly selected as the blank control group (0.5% carboxymethylcellulose)
and the other 76 rats were induced by Tripterygium wilfordii polyglycoside to establish the model of premature
ovarian dysfunction. After the model was established successfully, the rats were randomly divided into model
group (0.5% carboxymethylcellulose), 20 mg / kg res group (20 mg / kg RES), 40 mg / kg res group Res group
(40mg / kg RES) and 80mg / kg res group (80mg / kg RES). The levels of MDA, SOD, AMH, E2, LH, FSH,
ovary, PI3K / Akt / mTOR signaling pathways, pi13k, p-pi13k, Akt P-Akt, mTOR, p-mTOR, B-cell lymphoma
factor 2 (Bcl-2), Bax, Caspase-3, average optical density (Bax, Caspase-3, Bcl-2, p-Akt, p-mTOR). Compared
with the blank control group, the MDA level and SOD level of the model group were significantly increased.
Compared with the model group, the MDA level and SOD level of the rats in the res dose group decreased
significantly (P < 0.05). Compared with the blank control group, AMH and E2 levels of the model group were
significantly reduced, FSH and LH levels were significantly increased. Compared with the model group, the
levels of AMH, E2, FSH and LH in the res dose group were significantly higher, which was dose-dependent (P
< 0.05). Compared with the blank control group, the number of mature follicles and corpus luteum in the model
group decreased significantly, and the number of atresia follicles increased significantly (P < 0.05, in a
dose-dependent manner). Compared with the model group, the number of mature follicles, corpus luteum and
atresia follicles in the res dose group were significantly increased (P < 0.05). Compared with the blank control
group, the average optical density of Bax, Caspase-3, Bcl-2, p-Akt and p-mTOR in the model group were
significantly higher, and the average optical density of Bcl-2, p-Akt and p-mTOR were significantly lower (P <
0.05). Compared with the model group, the average optical density values of Bax, caspase-3 and Bcl-2, p-Akt
and p-mTOR in the res group were significantly lower, and the average optical density values of Bcl-2, p-Akt
and p-mTOR were significantly higher (P < 0.05). Compared with the blank control group, the expression levels
of Bax, caspase-3 and p-pi13k, p-Akt, p-mTOR and Bcl-2 in the model group were significantly higher (P <
0.05). Compared with the model group, the expression level of Bax and caspase-3 protein in the res dose group
was significantly lower, and the expression levels of p-pi13k, p-Akt, p-mTOR and bcl-2 protein were
significantly higher (P < 0.05). There was no significant difference in the expression of pi13k, Akt and mTOR
in each group (P > 0.05). Res can effectively increase the number of mature cells and corpus luteum, and
promote the recovery of ovarian function. In addition, res can play the role of ovarian protection by activating
pi3kaktmtor signaling pathway and antioxidant stress mechanism.

Published
2020-03-01