L-Selectin Transcription and Library Translation in Hemorrhagic Shock Rats

  • Suxing Zhao
Keywords: Hemorrhagic Shock, L-Selectin Expression, MRNA Expression Level, Polymerase Chain Reaction

Abstract

This paper aims to investigate the effects of l-selectin transcriptional and translational expressions on
the pathophysiological process of trauma and shock in hemorrhagic shock rats. In this paper, the tail blood of
rats was taken as the normal control, and the cervical artery ligation, clipping and intubation were used as the
experimental control to establish an animal model of hemorrhagic shock. The dynamic expression of l-selectin
on the surface of neutrophils in the acute stage of hemorrhagic shock in rats was detected by flow cytometry
using monoclonal antibody markers, and the semi-quantitative changes of l-selectin mRNA in leukocytes of rats
were detected by semi-quantitative reverse transcription polymerase chain reaction (rt-pcr). The experimental
results showed that there was no significant difference in the expression of l-selectin (mean fluorescence trace
number) at different time points on the surface of neutrophils in the normal control group (P>0.05). Compared
with the normal control group, the expression of mRNA on the cell surface and cytoplasm in the control group
was up-regulated, and the cell surface reached a peak at 3h, and the level remained high at 4h and 5h. The
mRNA increased progressively and reached the peak at 5h. Compared with the normal control group, both of the
experimental shock group were up-regulated. However, compared with the trauma group, there was a downward
trend after 3h. These results suggest that the up-regulated expression of neutrophils L- selectin can enhance the
adhesion response of leukocyte - endothelial cells, and help local wound healing, repair and fight infection. The
down-regulated expression of L- selectin in the shock group can reduce the excessive adhesion of leukocyte -
endothelial cells and impaction of capillaries, which can help the body to unclog the microcirculation and
improve the perfusion flow.

Published
2020-03-01